Extração de lipídios de microalgas: um novo método em escala de laboratório dentro de uma abordagem de biorrefinaria (fracionamento)
DOI:
https://doi.org/10.33414/rtyc.45.31-45.2022Palavras-chave:
Microalgae, Lipid, Fractioning, BiorefineryResumo
A extração e fracionamento de lipídios de microalgas tem sido objeto de estudo recorrente devido ao valor de mercado de algumas famílias de lipídios (carotenóides e xantofilas) e à oportunidade que os óleos de microalgas representam para a obtenção de biocombustíveis. A maioria dos métodos desenvolvidos para a extração total de lipídios de microalgas requer uma posterior separação das diferentes famílias (lipídios neutros, lipídios polares) para serem quantificados. Além disso, a maioria deles usa solventes tóxicos e caros.
No presente estudo, solventes menos tóxicos e amplamente utilizados na indústria, como hexano, acetona e etanol, foram testados em diferentes ordens para a extração de biomassa de microalgas de composição e teor lipídico diferentes (ou seja, três níveis diferentes de estresse nutricional ). Uma comparação dos métodos propostos por Bligh e Dyer, Folch e Hara e Radin também é apresentada. O novo método consegue a extração de lipídios totais de microalgas e a separação de famílias lipídicas simultaneamente.
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Copyright (c) 2022 Andrea Natalia Pila, Maria Carolina Cuello, Roberto Martin Schmitd, Ester Chamorro
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