Evaluación de Cepas Fermentativas en la Hidrólisis y Fermentación Simultáneas (SSF) de Cascarilla de Arroz para la Producción de Bioetanol
Keywords:
Bioethanol, Rice husk, Simultaneous Sacharification and Fermentation (SSF), EnzymesAbstract
The decrease in oil reserves worldwide and environmental problems caused by its use, lead to finding new renewable and less polluting alternatives.
This work presents the study of the behavior of both celobiosas and cellulase enzymes, together with strains of Saccharomyces cerevisiae, Candida kefyr, Candida tropicalis and Candida guilliermondii, specifically in the process of simultaneous hydrolysis and fermentation (SSF) conducted at 35 °C to produce bioethanol from the carbohydrates of rice husks.
This process seeks hydrolyze the cellulose and immediately utilize of fermentable sugars, overcoming the difficulty of the difference between the optimum temperature for the performance of enzyme (50°C) and yeast (25°C to 35°C). Rice husk was pretreated with an acid-alkaline sequence to achieve the separation of carbohydrates.
The best yields to bioethanol were obtained with Saccharomyces cerevisiae (68%) and Candida kefyr (75%).