Line detection algorithm applied to the improvement of LEXT OLYMPUS microscope calibration processes.
DOI:
https://doi.org/10.33414/rtyc.42.1-11.2021Keywords:
calibration slide, microscope, image processing, ConfocalAbstract
This paper presents an image processing method to optimize the calibration of Lext Olympus optical microscopes with templates. The proposed algorithm already works on the scales of Nikon autocollimators, which are similar to those present in the calibration templates of these microscopes. The algorithm was adapted to measure these templates and thus optimize the calibration.
Downloads
References
Caselles, J. y Mas, C. R. (2018). Microscopio confocal de reflexión de barrido mediante láser, LEXT (Olympus): una herramienta de medición y verificación de componentes claves de los equipos de medición y ensayo. Congreso Argentino de Microscopía SAMIC.
Brambilla, N., Brusa, D., Caselles, J., Mas, C. y Schurrer, C. (2019). Intercomparación en Mediciones Superficiales. Revista Tecnología y Ciencia; 35, 1-17.
Vicente y Oliva, J., Molpeceres Álvarez, C., Guarneiros, O. y García-Ballesteros Ramírez, J. (2008). Calibración de Microscopios Confocales. Anales de Ingeniería Mecánica.
Pfeifer, T.; Freudenberg, R., Dussler, G. y Bröcher, B. (2001). Quality control and process observation for the micro assembly process. Measurement. 30. 1-18.
De Chiffre, L., Carli, L. y Eriksen, R.S. (2011). Multiple height calibration artefact for 3D microscopy. CIRP Ann. Manuf. Technol. 60, 535–538.
Jensen, K.E., Weitz, D.A. y Spaepen, F. (2013). Note: A three-dimensional calibration device for the confocal microscope. Rev. Sci. Instrum, 84, 016108.
Walker, D. (2012). Notes on the potential vibration problems observed with two mirrorless digital cameras if used for photomicroscopy (Sony NEX-5N and Panasonic GF2), and the value of an electronic first curtain shutter option. http://www.microscopy-uk.org.uk/mag/artnov12/dw-SonyNEX5N.html
Webb, R.H. (1996). Confocal optical microscopy. Rep Prog Phys, 59(3), pp. 427-471.
Webb, R.H. (1999). Theoretical Basis of Confocal Microscopy. Meth. Enzymol. 307, pp. 3-20
Salerni, G. (2011). Uso de la microscopía confocal de reflectancia en dermatología. Dermatol. Argent., 17(3), pp. 230-235.
Claxton, N.S., Fellers, T.J. y Davidson, M.W. (2006). Laser scanning confocal microscopy. In Encyclopedia of Medical Devices and Instrumentation, 2nd ed.; Webster, J.G.; John Wiley & Sons, Inc., pp. 1-37.
Cheng, C., Wang, J., Leach, R.K., Lu, W., Liu, X. y Jiang, X. (2019). Corrected parabolic fitting for height extraction in confocal microscopy. Opt Express, 27(3), pp. 3682-3697.
Flesia, A. G., Ames, G., Bergues, G., Canali, L. y Schurrer, C. (2014). Sub- pixel straight lines detection for measuring through machine vision. Instrumentation and Measurement Technology Conference (I2MTC) Proceedings, 2014 IEEE International, pp. 402–406.
Schurrer, C., Flesia, A. G., Bergues, G., Ames, G. y Canali, L. (2014). Interfaz visual para un autocolimador nikon 6d mediante procesamiento de imágenes con precisión sub-pix́el: un caso de estudio. Revista Iberoamericana de Automática e Informática Industrial - RIAI, vol. 11, no. 3, pp. 327–336.
Bergues, G., Ames, G., Canali, L., Schurrer, C. y Flesia, A. G. (2014). External visual interface for a nikon 6d autocollimator. Instrumentation and Measurement Technology Conference (I2MTC) Proceedings, 2014 IEEE International pp. 35–39.
Bergues, G. J., Canali, L., Schurrer, C., y Flesia, A. (2015). Electronic interface with vignetting effect reduction for a Nikon 6b/6d autocollimator. Instrumentation and Measurement, IEEE Transactions on, no. 99.
Hecht, E. (2001). Optics (4th Edition), 4th ed. Addison Wesley.
Schafer, R. W. (2011). What is a savitzky-golay filter? [lecture notes]. Signal Processing, IEEE Transactions on, vol. 28, no. 4, pp. 111–117.
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Guillermo Juan Bergues, Clemar Schürrer, Juan Caselles, Nancy Brambilla
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
