Statistic optimization of Pseudomonas sp. culture media for biosurfactant production

Abstract

The aim of the present work is to obtain the optimal temperature and the optimal concentration of glycerol in culture media for biosurfactant production using a bacterial strain of the Pseudomonas sp. Optimization was carried out through response surface methodology using a face centered-central composite design, where analytical grade glycerol and temperature were the factors and biosurfactant production the response variable. We found a mathematical model based on experimental results that predicts the influence of the factors on biosurfactant production. According to the model, 7,6%v/v of glycerol and 25,9°C were the optimal values. Here, the predicted biosurfactant concentration would be 0,43g/l.